Rapid identification of Armillaria species by PCR-DGGE

Claudia M O Longa; Nicola La Porta

doi:10.1016/j.mimet.2014.09.002

Rapid identification of Armillaria species by PCR-DGGE

J Microbiol Methods. 2014 Dec:107:63-5. doi: 10.1016/j.mimet.2014.09.002. Epub 2014 Sep 16.

Authors

Claudia M O Longa¹, Nicola La Porta²

Affiliations

¹ Sustainable Agro-Ecosystems and Bioresources Department, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy. Electronic address: [email protected].
² Sustainable Agro-Ecosystems and Bioresources Department, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.

PMID: 25218110
DOI: 10.1016/j.mimet.2014.09.002

Abstract

The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armillaria species in Europe.

Keywords: Armillaria; DGGE; Fungi.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Armillaria / classification*
Armillaria / genetics*
DNA Fingerprinting / methods*
DNA, Fungal
DNA, Ribosomal Spacer
Polymerase Chain Reaction / methods*

Substances

DNA, Fungal
DNA, Ribosomal Spacer