Abstract
Corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) stimulate the secretion of beta-endorphin by human PBMC. It is shown here that peripheral blood B cells are responsible for the production of beta-endorphin after culture with CRF and AVP. The presence of CD14+ monocytes is, however, a prerequisite for the enhancing activity of CRF and AVP. The data presented here show that rIL-1 beta can replace CRF and AVP, whereas a mAb directed against IL-1 abrogates the response to CRF and AVP. These results indicate that IL-1 mediates the effect of CRF and AVP on beta-endorphin production by human PBMC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / physiology
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Arginine Vasopressin / pharmacology*
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B-Lymphocytes / physiology
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Binding, Competitive
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Cell Separation
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Cells, Cultured
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Corticotropin-Releasing Hormone / pharmacology*
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Humans
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Interleukin-1 / immunology
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Interleukin-1 / physiology*
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Leukocytes, Mononuclear / classification
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / physiology*
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Monocytes / physiology
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T-Lymphocytes / physiology
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beta-Endorphin / immunology
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beta-Endorphin / metabolism*
Substances
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Antibodies, Monoclonal
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Interleukin-1
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Arginine Vasopressin
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beta-Endorphin
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Corticotropin-Releasing Hormone