Molecular requirement for sterols in herpes simplex virus entry and infectivity

George A Wudiri; Suzanne M Pritchard; Hong Li; Jin Liu; Hector C Aguilar; Stacey D Gilk; Anthony V Nicola

doi:10.1128/JVI.01615-14

Molecular requirement for sterols in herpes simplex virus entry and infectivity

J Virol. 2014 Dec;88(23):13918-22. doi: 10.1128/JVI.01615-14. Epub 2014 Sep 17.

Authors

George A Wudiri¹, Suzanne M Pritchard², Hong Li³, Jin Liu⁴, Hector C Aguilar⁵, Stacey D Gilk⁶, Anthony V Nicola⁷

Affiliations

¹ Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA.
² Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
³ Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA Animal Disease Research Unit, USDA Agricultural Research Service, Washington State University, Pullman, Washington, USA.
⁴ School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington, USA.
⁵ Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA.
⁶ Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
⁷ Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA [email protected].

Abstract

Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24(-/-) cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cholesterol / metabolism*
Desmosterol / metabolism*
Herpesvirus 1, Human / physiology*
Virus Internalization*

Substances

Desmosterol
Cholesterol

Abstract

Publication types

MeSH terms

Substances

Grants and funding