Abstract
In this work, we demonstrated insulin signaling and the anti-inflammatory effects by the chloroform fraction of ethanolic extract of Nymphaea rubra flowers in TNF-α-induced insulin resistance in the rat skeletal muscle cell line (L6 myotubes) to dissect out its anti-hyperglycemic mechanism. N. rubra enhances the GLUT4-mediated glucose transport in a dose dependent manner and also increases the tyrosine phosphorylation of both IR-β and IRS-1, and the IRS-1 associated PI-3 kinase activity in TNF-α-treated L6 myotubes. Moreover, N. rubra decreases Ser(307) phosphorylation of IRS-1 by the suppression of JNK and NF-κB activation. In conclusion, N. rubra reverses the insulin resistance by the inhibition of c-Jun NH2-Terminal Kinase and Nuclear-κB.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / pharmacology*
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Cell Line
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Flowers / chemistry*
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Glucose / metabolism
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Glucose Transporter Type 4 / metabolism
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Hypoglycemic Agents / chemistry
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Hypoglycemic Agents / pharmacology*
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Insulin / metabolism
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Insulin Resistance*
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JNK Mitogen-Activated Protein Kinases / metabolism*
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Muscle Fibers, Skeletal / cytology
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Muscle Fibers, Skeletal / metabolism*
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NF-kappa B / metabolism*
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Nymphaea / chemistry*
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Rats
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Tumor Necrosis Factor-alpha / metabolism*
Substances
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Anti-Inflammatory Agents
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Glucose Transporter Type 4
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Hypoglycemic Agents
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Insulin
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NF-kappa B
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Plant Extracts
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Slc2a4 protein, rat
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Tumor Necrosis Factor-alpha
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JNK Mitogen-Activated Protein Kinases
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Glucose