Comparison of the effect of post-heparin and pre-heparin lipoprotein lipase and hepatic triglyceride lipase on remnant lipoprotein metabolism

Clin Chim Acta. 2015 Feb 2:440:193-200. doi: 10.1016/j.cca.2014.07.020. Epub 2014 Sep 17.

Abstract

Background: A comparison of post-heparin and pre-heparin plasma lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) on the metabolism of remnant lipoproteins (RLPs) has not been reported yet.

Methods: Healthy volunteers were injected with heparin for LPL and HTGL determination in the fasting (8:00) and postprandial (20:00) plasma on the same day. Plasma total cholesterol (TC), triglycerides (TG), LDL-C, HDL-C, small dense LDL (sdLDL)-C, remnant lipoprotein (RLP)-C, RLP-TG, the RLP-TG/RLP-C ratio, adiponectin and apoCIII were measured.

Results: LPL activity and concentration in the post-heparin plasma exhibited a significant inverse correlation with TG, RLP-C, RLP-TG, and RLP particle size estimated as RLP-TG/RLP-C ratio and sdLDL-C, and positively correlated with HDL-C. HTGL was only inversely correlated with HDL-C. LPL concentration in the pre-heparin plasma was also inversely correlated with the RLP-TG/RLP-C ratio and other lipoprotein parameters. Adiponectin was inversely correlated with RLP-TG/RLP-C ratio and apoC III was positively correlated with RLP-TG/RLP-C ratio, but not correlated with LPL activity.

Conclusion: LPL activity and concentration were inversely and significantly correlated with the particle size of RLP in both the post-heparin and pre-heparin plasma. Those results suggest that LPL concentration in pre-heparin plasma can take the place of LPL activity in the post-heparin plasma.

Keywords: Hepatic triglyceride lipase (HTGL); Lipoprotein lipase (LPL); Post-heparin plasma; Pre-heparin plasma; RLP-TG/RLP-C ratio; Remnant lipoproteins (RLP).

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Fasting
  • Female
  • Heparin / chemistry
  • Heparin / pharmacology*
  • Humans
  • Linear Models
  • Lipase / blood*
  • Lipase / metabolism
  • Lipoprotein Lipase / blood*
  • Lipoprotein Lipase / metabolism
  • Lipoproteins / blood
  • Lipoproteins / metabolism*
  • Male
  • Particle Size
  • Postprandial Period

Substances

  • Cholesterol, HDL
  • Cholesterol, LDL
  • Lipoproteins
  • Heparin
  • Lipase
  • Lipoprotein Lipase