Temporal insight into the natural generation of a new reassortant porcine influenza virus in a swine holding

Vet Microbiol. 2014 Nov 7;174(1-2):9-15. doi: 10.1016/j.vetmic.2014.08.026. Epub 2014 Sep 6.

Abstract

The influenza A virus (IAV) subtypes H1N1, H3N2 and H1N2 are the most prevalent subtypes in swine in Italy. Reassortant influenza A viruses subtypes in swine appeared in European pig population. In particular reassortant viruses carrying genome segment from the pandemic H1N1 (H1N1pdm) are reported in many European countries, including Italy. In a 1000 sows farrow-to feeder farm, in Northern Italy, we isolated 10 IAVs from recurrent episodes of respiratory disease in 45-70 days-old pigs from September 2012 to June 2013. The antigenic and genetic characterization of the swine IAV isolates showed the contemporary circulation of H1N1 avian-like and H1N1pdm strains in the first outbreak. The analysis of the viruses isolated subsequently showed the circulation of H1N1pdm IAV and then the establishment of a new previously undescribed H1N1 reassortant strain with a pandemic derived hemagglutinin gene and all the other seven segments of swine H1N1 avian-like lineage.

Keywords: H1N1; Reassortant; Swine influenza A virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Epidemiological Monitoring / veterinary*
  • Influenza A Virus, H1N1 Subtype / genetics*
  • Italy / epidemiology
  • Molecular Sequence Data
  • Orthomyxoviridae Infections / epidemiology
  • Orthomyxoviridae Infections / veterinary*
  • Real-Time Polymerase Chain Reaction / veterinary
  • Reassortant Viruses / genetics*
  • Respiration Disorders / epidemiology
  • Respiration Disorders / veterinary*
  • Respiration Disorders / virology
  • Sequence Analysis, DNA / veterinary
  • Swine
  • Swine Diseases / epidemiology*
  • Swine Diseases / virology*

Associated data

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