Intravenous immunoglobulin G improves neurobehavioral and histological outcomes after traumatic brain injury in mice

J Neuroimmunol. 2014 Nov 15;276(1-2):112-8. doi: 10.1016/j.jneuroim.2014.08.626. Epub 2014 Sep 6.

Abstract

Intravenous immunoglobulin (IVIG) may improve neuroinflammation after traumatic brain injury (TBI). IVIG administration after TBI improved rotarod latencies over the first 7 days (p=0.039) and water maze latencies over 29-32 days (p=0.027), decreased F4/80-positive cells at 2 (p=0.001) and 7 days (p<0.001), decreased Fluoro-Jade B-positive cells (p=0.020), increased NeuN-positive cells (p=0.014), decreased IL-6 production at 4 (p=0.032) and 24h (p=0.023), and decreased blood-brain barrier breakdown by IgG extravasation (p=0.001) and brain edema (p=0.006); however, TNF-α concentration was unchanged. IVIG administration was associated with long-term neurobehavioral and histological improvement through modulation of neuroinflammation and blood-brain barrier permeability in a murine TBI model.

Keywords: Blood–brain barrier (BBB); Intravenous immunoglobulin (IVIG); Long-term outcome; Murine model; Neuroinflammation; Traumatic brain injury (TBI).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation / metabolism
  • Blood-Brain Barrier / drug effects
  • Blood-Brain Barrier / physiopathology
  • Brain / drug effects
  • Brain / metabolism*
  • Brain Edema / drug therapy
  • Brain Edema / etiology
  • Brain Injuries / complications
  • Brain Injuries / drug therapy*
  • Brain Injuries / pathology*
  • Cell Count
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Fluoresceins
  • Immunoglobulins, Intravenous / pharmacology
  • Immunoglobulins, Intravenous / therapeutic use*
  • Interleukin-6 / metabolism
  • Male
  • Maze Learning / drug effects*
  • Mice
  • Mice, Inbred C57BL
  • Microglia / drug effects
  • Microglia / metabolism
  • Motor Activity / drug effects*
  • Phosphopyruvate Hydratase / metabolism
  • Reaction Time / drug effects
  • Time Factors

Substances

  • Antigens, Differentiation
  • Fluoresceins
  • Immunoglobulins, Intravenous
  • Interleukin-6
  • fluoro jade
  • monocyte-macrophage differentiation antigen
  • Phosphopyruvate Hydratase