Pressure-controlled reperfusion improves postischemic recovery of LV-hypertrophied rat hearts

Angiology. 1989 Jun;40(6):574-80. doi: 10.1177/000331978904000610.

Abstract

The influence of pressure-controlled postischemic reperfusion (Rp) on functional and metabolic parameters in hearts of sham-operated rats and hypertrophied hearts of rats with aortic constriction were studied. Hypertrophied hearts are considered to be more susceptible to ischemia. The hearts were perfused in the Langendorff-technique for thirty minutes at 35 degrees C with Krebs-Henseleit bicarbonate buffer at a perfusion pressure (PP) of 75 mmHg and for five minutes at 15 degrees C with St. Thomas' Hospital cardioplegic solution at a PP of 60 mmHg. After a period of global ischemia of forty minutes' duration at 15 degrees C, reperfusion was started either abruptly (aRp: PP 75 mmHg immediately) or gently (gRp: PP 75 mmHg within thirty minutes); it lasted for forty-five minutes. Intraventricular peak systolic pressure (ISP) was monitored and energy-rich compounds (ATP, ADP, AMP, CrP, free Cr) were analyzed. In normal hearts, metabolic recovery was not affected by the mode of reperfusion, but functional recovery (ISP) averaged 88% of the preischemic control value after gRp as compared with 73% after aRp. In hypertrophied hearts, gentle reperfusion ameliorated both metabolic and functional recovery. At forty-five minute recovery, CrP averaged 5.1 mumol/g ww after aRp and 6.6 mumol/g ww after gRp (p less than 0.01), and ISP amounted to 73% of the preischemic control after aRp and to 85% after gRp.

MeSH terms

  • Adenine Nucleotides / metabolism
  • Animals
  • Cardiomegaly / metabolism
  • Cardiomegaly / physiopathology*
  • Cardiomegaly / surgery
  • Male
  • Myocardial Reperfusion / methods*
  • Phosphocreatine / metabolism
  • Rats
  • Rats, Inbred Strains

Substances

  • Adenine Nucleotides
  • Phosphocreatine