Genotypic and phenotypic detection of efflux pump in Rhodococcus equi

Braz J Microbiol. 2014 Aug 29;45(2):661-5. doi: 10.1590/s1517-83822014000200040. eCollection 2014.

Abstract

The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi.

Keywords: Rhodococcus equi; efflux pump; ethidium bromide; multidrug resistance; vapA.

MeSH terms

  • Biological Transport, Active
  • DNA, Bacterial / genetics
  • Ethidium / metabolism
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism*
  • Polymerase Chain Reaction
  • Rhodococcus equi / genetics*
  • Rhodococcus equi / metabolism*

Substances

  • DNA, Bacterial
  • Membrane Transport Proteins
  • Ethidium