Extracellular vesicles as enhancers of virus vector-mediated gene delivery

Zachary Fitzpatrick; Bence György; Johan Skog; Casey A Maguire

doi:10.1089/hum.2014.082

Extracellular vesicles as enhancers of virus vector-mediated gene delivery

Hum Gene Ther. 2014 Sep;25(9):785-6. doi: 10.1089/hum.2014.082.

Authors

Zachary Fitzpatrick¹, Bence György, Johan Skog, Casey A Maguire

Affiliation

¹ 1 Department of Neurology, The Massachusetts General Hospital , and Neuroscience Program, Harvard Medical School, Boston, MA 02129.

Abstract

Extracellular vesicles (EVs) being released from two adjacent adeno-associated virus serotype 1 (AAV1)-producing 293T cells are shown by electron microscopy. We have shown that AAV vectors can associate with EVs and enter the media. Furthermore, we have recently reported that EV-associated AAV has robust gene delivery and antibody evasion properties in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dependovirus / ultrastructure*
Gene Transfer Techniques*
Genetic Vectors / genetics*
Microscopy, Electron / methods*
Transport Vesicles / genetics*
Transport Vesicles / virology*