A method for evaluating the level of soluble β-amyloid(1-40/1-42) in Alzheimer's disease based on the binding of gelsolin to β-amyloid peptides

Angew Chem Int Ed Engl. 2014 Nov 17;53(47):12832-5. doi: 10.1002/anie.201405001. Epub 2014 Sep 22.

Abstract

In the present work, a new electrochemical strategy for the sensitive and specific detection of soluble β-amyloid Aβ(1-40/1-42) peptides in a rat model of Alzheimer's disease (AD) is described. In contrast to previous antibody-based methods, β-amyloid(1-40/1-42) was quantified based on its binding to gelsolin, a secretory protein present in the cerebrospinal fluid (CSF) and plasma. The level of soluble β-amyloid peptides in the CSF and various brain regions were found with this method to be lower in rats with AD than in normal rats.

Keywords: Alzheimer’s disease; aggregation; biosensors; electrochemistry; β-amyloid peptides.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / metabolism*
  • Amyloid beta-Peptides / analysis*
  • Amyloid beta-Peptides / chemistry
  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Binding Sites
  • Electrochemical Techniques / methods*
  • Gelsolin / metabolism*
  • Rats
  • Solubility

Substances

  • Amyloid beta-Peptides
  • Gelsolin