Vitamin B1 deficiency inhibits the increased conversion of tryptophan to nicotinamide in severe food-restricted rats

Biosci Biotechnol Biochem. 2015;79(1):103-8. doi: 10.1080/09168451.2014.962473. Epub 2014 Sep 25.

Abstract

The conversion of tryptophan (Trp) → nicotinamide (Nam) is an important pathway for supplying vitamin niacin. We reported the following two phenomena: (1) severe food restriction led to an increase in the Trp → Nam conversion compared with free-access control group; (2) the conversion of Trp → Nam is also increased by vitamin B1 deficiency compared with free-access control group. The present study was done to clarify whether or not a true reason about an increase in the Trp → Nam conversion is a vitamin B1 deficiency or severe food restriction. The present results showed that vitamin B1 deficiency suppressed the increased conversion of Trp → Nam induced by severe food restriction, probably by suppressing 3-hydroxyanthranilic acid 3,4-dioxygenase protein synthesis in liver.

Keywords: food restriction; nicotinamide; rat; tryptophan; vitamin B1.

MeSH terms

  • 3-Hydroxyanthranilate 3,4-Dioxygenase / biosynthesis
  • 3-Hydroxyanthranilic Acid / metabolism
  • Animals
  • Food Deprivation*
  • Liver / metabolism*
  • Liver / pathology
  • Male
  • Niacin / metabolism
  • Niacinamide / metabolism*
  • Protein Biosynthesis
  • Rats
  • Rats, Wistar
  • Thiamine / metabolism*
  • Tryptophan / metabolism*
  • Vitamin B Deficiency / metabolism*
  • Vitamin B Deficiency / pathology

Substances

  • 3-Hydroxyanthranilic Acid
  • Niacinamide
  • Niacin
  • Tryptophan
  • 3-Hydroxyanthranilate 3,4-Dioxygenase
  • Thiamine