MiR-30a inhibits osteolysis by targeting RunX2 in giant cell tumor of bone

Biochem Biophys Res Commun. 2014 Oct 10;453(1):160-5. doi: 10.1016/j.bbrc.2014.09.076. Epub 2014 Sep 27.

Abstract

RunX2 has been identified to crucially regulate the osteolysis in giant cell tumor of bone. MiR-30a is an intronic miRNA identified as tumor suppressor, but little is known about its role in giant tumor cell of bone. In our research, we reported miR-30a was down-regulated in GCT whereas RunX2 was highly expressed. Further research proved that miR-30a can regulate the expression of RunX2 by binding to its 3'-UTR, which influence the osteoclast differentiation and osteolysis formation. Thus, these results suggest that miR-30a could directly target RunX2 and participate in osteolysis in GCT.

Keywords: Giant cell tumor of bone; Osteoclast differentiation; RunX2; miR-30a-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Base Sequence
  • Bone Neoplasms / genetics*
  • Bone Neoplasms / metabolism
  • Bone Neoplasms / pathology*
  • Core Binding Factor Alpha 1 Subunit / genetics*
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Giant Cell Tumor of Bone / genetics*
  • Giant Cell Tumor of Bone / metabolism
  • Giant Cell Tumor of Bone / pathology*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Sequence Data
  • Osteoclasts / metabolism
  • Osteoclasts / pathology
  • Osteolysis / genetics*
  • Osteolysis / pathology
  • Osteolysis / prevention & control*
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • 3' Untranslated Regions
  • Core Binding Factor Alpha 1 Subunit
  • MIRN30b microRNA, human
  • MicroRNAs
  • RNA, Neoplasm
  • RUNX2 protein, human