Unaltered regulatory B-cell frequency and function in patients with multiple sclerosis

Clin Immunol. 2014 Dec;155(2):198-208. doi: 10.1016/j.clim.2014.09.011. Epub 2014 Sep 28.

Abstract

Multiple sclerosis (MS) is a chronic disease of the central nervous system (CNS) typically characterized by the recruitment of T cells into the CNS. However, certain subsets of B cells have been shown to negatively regulate autoimmune diseases and some data support a prominent role for B cells in MS physiopathology. For B cells in MS patients we analyzed subset frequency, cytokine secretion ability and suppressive properties. No differences in the frequencies of the B-cell subsets or in their ability to secrete cytokines were observed between MS and healthy volunteers (HV). Prestimulated B cells from MS patients also inhibited CD4(+)CD25(-) T cell proliferation with a similar efficiency as B cells from HV. Altogether, our data show that, in our MS patient cohort, regulatory B cells have conserved frequency and function.

Keywords: B cells; Multiple sclerosis; Regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antigens, Surface / metabolism
  • B-Lymphocytes, Regulatory / drug effects
  • B-Lymphocytes, Regulatory / immunology*
  • B-Lymphocytes, Regulatory / metabolism
  • CD40 Ligand / metabolism
  • Case-Control Studies
  • Cell Communication / immunology
  • Cytokines / biosynthesis
  • Female
  • Humans
  • Immunophenotyping
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Male
  • Middle Aged
  • Multiple Sclerosis / diagnosis
  • Multiple Sclerosis / immunology*
  • Multiple Sclerosis / metabolism
  • Oligonucleotides / immunology
  • Oligonucleotides / pharmacology
  • Phenotype
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism
  • Young Adult

Substances

  • Antigens, Surface
  • Cytokines
  • Oligonucleotides
  • CD40 Ligand