Time-resolved crystallography using the Hadamard transform

Nat Methods. 2014 Nov;11(11):1131-4. doi: 10.1038/nmeth.3139. Epub 2014 Oct 5.

Abstract

We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography, X-Ray / methods*
  • Plant Proteins / chemistry*
  • Protein Conformation
  • Signal-To-Noise Ratio
  • Time Factors

Substances

  • Plant Proteins
  • thaumatin protein, plant

Associated data

  • PDB/4C3C