Nuclear transfer and transgenesis in the pig

Methods Mol Biol. 2015:1222:37-59. doi: 10.1007/978-1-4939-1594-1_4.

Abstract

Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, we introduce our routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, SCNT using in vitro matured oocytes, and laparoscopic embryo transfer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified*
  • Anti-Bacterial Agents / pharmacology
  • Embryo Transfer / methods*
  • Female
  • Gene Targeting / methods*
  • Gene Transfer Techniques
  • In Vitro Oocyte Maturation Techniques / methods*
  • Laparoscopy / methods
  • Nuclear Transfer Techniques* / instrumentation
  • Pregnancy
  • Sus scrofa / genetics*
  • Transfection / methods
  • Workflow

Substances

  • Anti-Bacterial Agents