A DNA-based molecular probe for optically reporting cellular traction forces

Nat Methods. 2014 Dec;11(12):1229-32. doi: 10.1038/nmeth.3145. Epub 2014 Oct 12.

Abstract

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion / physiology*
  • Cells, Cultured
  • DNA Probes* / chemistry
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Focal Adhesions / physiology*
  • Mechanotransduction, Cellular / physiology*
  • Mice
  • Microscopy, Fluorescence

Substances

  • DNA Probes