Polyelectrolyte complexes of poly[(2-dimethylamino) ethyl methacrylate]/chondroitin sulfate obtained at different pHs: I. Preparation, characterization, cytotoxicity and controlled release of chondroitin sulfate

Letícia C Bonkovoski; Alessandro F Martins; Ismael C Bellettini; Francielle P Garcia; Celso V Nakamura; Adley F Rubira; Edvani C Muniz

doi:10.1016/j.ijpharm.2014.10.017

Polyelectrolyte complexes of poly[(2-dimethylamino) ethyl methacrylate]/chondroitin sulfate obtained at different pHs: I. Preparation, characterization, cytotoxicity and controlled release of chondroitin sulfate

Int J Pharm. 2014 Dec 30;477(1-2):197-207. doi: 10.1016/j.ijpharm.2014.10.017. Epub 2014 Oct 12.

Authors

Letícia C Bonkovoski¹, Alessandro F Martins², Ismael C Bellettini³, Francielle P Garcia⁴, Celso V Nakamura⁴, Adley F Rubira¹, Edvani C Muniz⁵

Affiliations

¹ Grupo de Materiais Poliméricos e Compósitos, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, 87020-900 Maringá, PR, Brazil.
² Grupo de Materiais Poliméricos e Compósitos, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, 87020-900 Maringá, PR, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, 85660-000 Dois Vizinhos, PR, Brazil.
³ Laboratório Polissol, Departamento de Química, Universidade Federal de Santa Catarina UFSC, 88040-900 Florianópolis, SC, Brazil.
⁴ Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá UEM, Av. Colombo 5790, 87020-900 Maringá, PR, Brazil.
⁵ Grupo de Materiais Poliméricos e Compósitos, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, 87020-900 Maringá, PR, Brazil. Electronic address: [email protected].

PMID: 25311176
DOI: 10.1016/j.ijpharm.2014.10.017

Abstract

For the first time, polyelectrolyte complex based on poly[(2-dimethylamino) ethyl methacrylate] (PDMAEMA) and chondroitin sulfate (CS) was prepared. The properties of novel material and precursors were investigated by WAXS, FTIR, TGA, SEM and DLS analysis. The PDMAEMA/CS PECs presented hydrophilic-hydrophobic transition at pHs 6.0, 7.0 and 8.0 whereas the non-complexed PDMAEMA showed such a transition at pH 8.0 and not at pHs 6.0 and 7.0. Studies of CS release from PECs at pHs 6 and 8 confirmed that the samples possess the potential to release the CS in alkaline and not in acidic conditions. Since PECs are thermo-responsive due to the reduction of LCST caused by the increase in pH, the release of CS was dependent on temperature and pH factors. Cytotoxicity assays using healthy VERO cells showed that the complexation between CS and PDMAEMA increased the PECs' biocompatibility related to PDMAEMA. However, the biocompatibility depends on the amount of CS present in the PECs.

Keywords: Chondroitin sulfate; Cytotoxicity; Poly[(2-dimethylamino) ethyl methacrylate]; Polyelectrolyte complexes; release.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Survival / drug effects
Chlorocebus aethiops
Chondroitin Sulfates / chemistry*
Chondroitin Sulfates / toxicity
Drug Compounding*
Drug Liberation
Drug Stability
Electrolytes
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Methacrylates / chemistry*
Methacrylates / toxicity
Nylons / chemistry*
Nylons / toxicity
Solubility
Surface Properties
Temperature
Vero Cells

Substances

Electrolytes
Methacrylates
Nylons
poly(2-(dimethylamino)ethyl methacrylate)
Chondroitin Sulfates