An orthogonal photolabile linker for the complete "on-support" synthesis/fast deprotection/hybridization of RNA

Chem Commun (Camb). 2014 Dec 11;50(95):15063-6. doi: 10.1039/c4cc07153k.

Abstract

The preparation of a polystyrene solid support decorated with a photolabile linker is described. The entire post-synthetic processing of RNA can be carried out in the solid phase in a minimum amount of time. The deprotected RNA is available for "on-support" hybridization and photolysis releases siRNA duplexes under mild, neutral conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Knockdown Techniques
  • HeLa Cells
  • Humans
  • Luciferases, Firefly / genetics
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemistry
  • Photolysis
  • Polystyrenes / chemistry
  • RNA / chemistry*
  • Ultraviolet Rays

Substances

  • Oligonucleotides
  • Polystyrenes
  • RNA
  • Luciferases, Firefly