Abstract
The radiation inactivation technique has been used to estimate the molecular size of the 5-HT3 receptor binding site labelled by [3H]zacopride, in comparison with that of the 5-HT1A receptor binding site labelled by [3H]8-OH-DPAT, in rat cortical membranes. The calculated molecular weight of the 5-HT3 site: 35.4 +/- 2.2 kDa (mean +/- S.E.M., n = 4) was significantly less than that of the 5-HT1A site: 62.9 +/- 1.8 kDa (mean +/- S.E.M., n = 4) and of other 5-HT1 and 5-HT2 receptors of the G-protein coupled family. These data further support that the 5-HT3 receptor is not coupled to G-proteins in the rat brain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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8-Hydroxy-2-(di-n-propylamino)tetralin
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Animals
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Benzamides / pharmacology
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Bridged Bicyclo Compounds / pharmacology
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Bridged Bicyclo Compounds, Heterocyclic*
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Cerebral Cortex / drug effects
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Cerebral Cortex / metabolism
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Cerebral Cortex / radiation effects
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In Vitro Techniques
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Kinetics
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Male
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Molecular Weight
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Rats
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Rats, Inbred Strains
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Receptors, Serotonin / analysis
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Receptors, Serotonin / metabolism*
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Receptors, Serotonin / radiation effects
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Tetrahydronaphthalenes / metabolism
Substances
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Benzamides
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Bridged Bicyclo Compounds
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Bridged Bicyclo Compounds, Heterocyclic
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Receptors, Serotonin
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Tetrahydronaphthalenes
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8-Hydroxy-2-(di-n-propylamino)tetralin
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zacopride