Retrospective screening of population was carried out in the Uzbek SSR in 3 physiographic regions, which formerly represented active epidemic foci of visceral leishmaniasis. Seroepidemiologic examination of 8652 persons showed satisfactory results concerning the disease at present. Despite low seropositive level of healthy population in general (0.38%), a correlation was detected between the number of positive indirect immunofluorescence results and the incidence rate of visceral leishmaniasis in this territory. In future it is recommended to perform regular retrospective screening of population in formerly endemic regions with a view to take timely preventive measures.