Fraction of immature granulocytes reflects severity but not mortality in sepsis

Scand J Clin Lab Invest. 2015 Jan;75(1):36-43. doi: 10.3109/00365513.2014.965736. Epub 2014 Oct 24.

Abstract

Objective: In order to validate immature granulocytes as a universal biomarker, we have compared the clinical relevance of the proportion of immature granulocytes (IG%), measured using Sysmex XE-2100, with other biomarkers (white blood cell, C-reactive protein, lactate and procalcitonin).

Methods: This single center, retrospective study included 184 patients with sepsis admitted to an emergency department. Patients were classified into two groups: Uncomplicated sepsis and complicated sepsis (severe sepsis and septic shock). IG% and other biomarkers were evaluated and compared for predicting sepsis severity, overt disseminated intravascular coagulation (DIC) and 28 day mortality.

Results: In multivariate analysis, only IG% (odd ratio [OR] 2.530, p = 0.004) and lactate (OR 4.500, p < 0.001) could discriminate between complicated and uncomplicated sepsis. The optimal cut-off value for IG% and lactate was 0.5% and 2.0 mmol/L, respectively. In subgroup analyses of complicated sepsis, IG% was related to overt DIC. However, no single biomarker could predict 28-day mortality.

Conclusions: Given that IG% reflected sepsis severity and overt DIC without additional cost, IG% could be a useful biomarker in patients with sepsis. However, there is a limitation for using it as a novel biomarker in sepsis due to the disability of prediction for 28-day mortality.

Keywords: Analyzer; C-reactive protein; DIC; biomarker; lactate; procalcitonin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Female
  • Granulocytes / immunology*
  • Humans
  • Leukocyte Count
  • Male
  • Middle Aged
  • Multivariate Analysis
  • ROC Curve
  • Sepsis / immunology
  • Sepsis / mortality*
  • Sepsis / pathology
  • Severity of Illness Index
  • Survival Analysis