Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex

G C Miescher; M Schreyer; H R MacDonald

doi:10.1016/0165-2478(89)90122-3

Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex

Immunol Lett. 1989 Dec;23(2):113-8. doi: 10.1016/0165-2478(89)90122-3.

Authors

G C Miescher¹, M Schreyer, H R MacDonald

Affiliation

¹ Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.

PMID: 2534389
DOI: 10.1016/0165-2478(89)90122-3

Abstract

We describe the production of a rat monoclonal antibody, 17A2, that detects the T cell receptor-associated CD3 molecular complex. 17A2 cross-competes with a CD3 epsilon-specific reagent and similarly stimulates IL-2 production by T cells. Immunohistological and cell separation applications are shown.

MeSH terms

Animals
Antibodies, Monoclonal*
Antigens, Differentiation, T-Lymphocyte / immunology*
CD3 Complex
Female
Immunohistochemistry
Interleukin-2 / metabolism
Mice
Mice, Inbred C57BL
Rats
Receptors, Antigen, T-Cell / immunology*
T-Lymphocytes / immunology
Thymus Gland / immunology

Substances

Antibodies, Monoclonal
Antigens, Differentiation, T-Lymphocyte
CD3 Complex
Interleukin-2
Receptors, Antigen, T-Cell