Hemostatic efficacy of EVARREST™, Fibrin Sealant Patch vs. TachoSil® in a heparinized swine spleen incision model

J Invest Surg. 2014 Dec;27(6):360-5. doi: 10.3109/08941939.2014.941444.

Abstract

Background: First-generation single-component hemostats such as oxidized regenerated cellulose (ORC), fibrin, collagen, and gelatin have evolved into second and third generations of combination hemostats.

Objective: This study compares two FDA approved products, EVARREST™, Fibrin Sealant Patch, a hemostat comprised of a matrix of nonwoven polyglactin 910 embedded in ORC coated with human fibrinogen and thrombin to TachoSil® medicated sponge, an equine collagen pad coated with human fibrinogen and thrombin.

Materials and methods: Swine were anticoagulated with heparin to 3X their baseline activated clotting time and a 15 mm long × 3 mm deep incision was made to create a consistent moderate bleeding pattern. Test material was then applied to the wound site and compressed manually for 3 min with just enough pressure to prevent continued bleeding. Hemostatic effectiveness was evaluated at 3 min and 10 min.

Results: At 3 min, the hemostasis success rate was 86% in the EVARREST™ group and 0% in the TachoSil® group, p < .0001. The overall success rate at 10 min was 100% with EVARREST™ and 4% with TachoSil®, p < .0001. Adhesive failure, in which the test material did not stick to the tissue, occurred in 96% of TachoSil® sites. In contrast, 100% of the EVARREST™ applications adhered to the test site.

Conclusions: EVARREST™, Fibrin Sealant Patch demonstrated greater wound adhesion and more effective hemostasis than TachoSil®. Adhesive failure was the primary failure mode for TachoSil® in this model.

Keywords: EVARRESTTM, Fibrin Sealant Patch; ORC; TachoSil®; hemostasis; oxidized regenerated cellulose; polyglactin 910.

MeSH terms

  • Animals
  • Anticoagulants / administration & dosage*
  • Anticoagulants / pharmacology
  • Blood Coagulation / drug effects
  • Cellulose / pharmacology
  • Cellulose / therapeutic use
  • Collagen / pharmacology
  • Collagen / therapeutic use
  • Drug Combinations
  • Female
  • Fibrin Tissue Adhesive / pharmacology
  • Fibrin Tissue Adhesive / therapeutic use*
  • Fibrinogen / pharmacology
  • Fibrinogen / therapeutic use*
  • Hemostasis, Surgical / methods*
  • Hemostatics / pharmacology
  • Hemostatics / therapeutic use*
  • Heparin / administration & dosage*
  • Heparin / pharmacology
  • Male
  • Models, Animal
  • Spleen / surgery*
  • Swine
  • Thrombin / pharmacology
  • Thrombin / therapeutic use*
  • Time Factors

Substances

  • Anticoagulants
  • Drug Combinations
  • Fibrin Tissue Adhesive
  • Hemostatics
  • TachoSil
  • Fibrinogen
  • Cellulose
  • Heparin
  • Collagen
  • Thrombin