Laser-assisted generation of human induced pluripotent stem cells

Yang Liu; Dongmei Wu; Donglin Lao; Cory Peterson; Kristi A Hohenstein Elliott; Evan Y Snyder

doi:10.1002/9780470151808.sc04a07s31

Laser-assisted generation of human induced pluripotent stem cells

Curr Protoc Stem Cell Biol. 2014 Nov 3:31:4A.7.1-15. doi: 10.1002/9780470151808.sc04a07s31.

Authors

Yang Liu¹, Dongmei Wu, Donglin Lao, Cory Peterson, Kristi A Hohenstein Elliott, Evan Y Snyder

Affiliation

¹ Stem Cell Research Center and Core Facility, Sanford-Burnham Medical Research Institute, La Jolla, California.

PMID: 25366899
DOI: 10.1002/9780470151808.sc04a07s31

Abstract

This unit describes a procedure for generating human induced pluripotent stem cells (hiPSCs) using the Laser-Enabled Analysis and Processing (LEAP®) system, which combines high-throughput cell imaging with laser-mediated cell manipulation. Use of this system should not only improve the quality and uniformity of hiPSCs produced, but ultimately enable a more rapid, efficient, high-throughput, and automated production process.

Keywords: automated; high-throughput; iPSC generation; laser-assisted.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Cellular Reprogramming Techniques / methods*
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism*
Lasers*

Grants and funding

P30-NS057096/NS/NINDS NIH HHS/United States