Two-photon photolysis combined with a kilobeam array scanner to probe calcium signaling in cardiomyocytes

Benjamin Sauer; Martin Oberhofer; Peter Lipp; Lars Kaestner

doi:10.1101/pdb.prot077008

Two-photon photolysis combined with a kilobeam array scanner to probe calcium signaling in cardiomyocytes

Cold Spring Harb Protoc. 2014 Nov 3;2014(11):1170-4. doi: 10.1101/pdb.prot077008.

Authors

Benjamin Sauer¹, Martin Oberhofer¹, Peter Lipp¹, Lars Kaestner¹

Affiliation

¹ Institute for Molecular Cell Biology and Research Center for Molecular Imaging and Screening, School of Medicine, Saarland University, 66421 Homburg/Saar, Germany.

PMID: 25368304
DOI: 10.1101/pdb.prot077008

Abstract

Photorelease of caged compounds allows a fast and defined intracellular increase in calcium (Ca(2+)) or other biologically relevant substances or molecules without impinging on other cellular functions. In particular, two-photon photolysis (2PP) allows a spatially restricted uncaging in sub-femtoliter volumes. Here, we describe how to combine 2PP with a confocal kilobeam array scanner and provide an example where Ca(2+) is released in an isolated cardiac myocyte.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium Signaling*
Cytological Techniques / methods*
Microscopy, Fluorescence, Multiphoton / methods*
Myocytes, Cardiac / physiology*
Optical Imaging / methods*
Photolysis