Injection of RCAS viruses is highly customizable to the desired target tissue. RCAS viruses can be delivered into mice in vivo by injection of virus-producing cells or by injection of concentrated virus. When cells are injected, they persist for several days, continuously producing virus. Typically the decision of whether to inject virus-producing cells or concentrated virus is determined by the volume that can be reliably injected into a given tissue and the age of the animal when the virus delivery is performed. This general protocol describes the intraperitoneal injection of RCAS-expressing cells into mice and discusses the circumstances in which the injection of concentrated virus is preferred.
© 2014 Cold Spring Harbor Laboratory Press.