Development of biodegradable polymers-based scaffolds for tissue engineering is a promising trend in bioengineering. The electrospun scaffolds from poly(3-hydroxybutyrate) (PHB) were produced using different additives that changed the physical and chemical characteristics of the products. As a result, the construct consisting of interwoven threads of different diameter (0.8-3.4 mm) were obtained, the smallest diameter was observed in the threads from the PHB using tetrabutilammonium iodide (TBAI) and titanium oxide II (TiO2) as additives. Mesenchymal stem cells (MSC) were cultivated on the scaffolds for the biocompatibility evaluation of obtained materials. Cells viability was determined by the XTT assay test. It was shown that the scaffold from the interwoven threads of lowest diameter is most favorable for MSC growth in comparison with the polymer film and scaffolds from the threads of larger diameter. Thus, it was shown that the biocompatibility of electrospun PHB scaffolds depended on their microstructure. The obtained data can be used for development of scaffolds for tissue engineering.
Konstruirovanie matriksov dlia tkanevoĭ inzhenerii iz biorazlagaemykh polimerov – perspektivnoe napravlenie v bioinzhenerii. V nastoiashcheĭ rabote byli izgotovleny matriksy iz biopolimera poli-3-oksibutirata (POB) metodom élektroformovaniia s ispol'zovaniem razlichnykh dobavok, izmeniaiushchikh fiziko-khimicheskie kharakteristiki izdeliĭ. V rezul'tate poluchilis' struktury, sostoiashchie iz perepletennykh niteĭ razlichnogo diametra (0,8-3,4 mkm); naimen'shiĭ diametr nabliudalsia u niteĭ iz POB s ispol'zovaniem v kachestve dobavok tetrabutilammoniĭ ĭodida (TBAI) i oksida titana II (TiO2). Dlia otsenki biosovmestimosti poluchennykh materialov na étikh matriksakh kul'tivirovali mezenkhimal'nye stvolovye kletki (MSK). Naibolee blagopriiatnoĭ podlozhkoĭ dlia rosta kletok iavliaiutsia matriksy iz perepletennykh niteĭ samogo nizkogo diametra, v sravnenii s polimernoĭ plenkoĭ i s matriksami iz niteĭ bol'shego diametra. Takim obrazom, byla pokazana zavisimost' biosovmestimosti polimernykh matriksov, poluchennykh metodom élektroformovaniia, ot ikh mikrostruktury, chto mozhet byt' ispol'zovano v tkanevoĭ inzhenerii.
Keywords: MSC; biocompatibility; electrospinning; poly(3-hydroxybutyrate).