Molecular cloning of a feline leukemia provirus integrated adjacent to the c-myc gene in a feline T-cell leukemia cell line and the unique structure of its long terminal repeat

Virology. 1989 Apr;169(2):458-61. doi: 10.1016/0042-6822(89)90172-4.

Abstract

This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DNA of the FT-1 cell line. The c-myc rearrangement in FT-1 was due to direct integration of the FeLV provirus genome immediately upstream of the c-myc gene in the opposite transcriptional orientation. Nucleotide sequencing showed that the LTR of this provirus had three copies of an enhancer-like sequence, unlike the sequences of FeLVs reported previously, which have only a single copy of this enhancer-like sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Transformation, Viral
  • Cloning, Molecular
  • DNA, Viral / genetics*
  • Leukemia Virus, Feline / genetics*
  • Leukemia, T-Cell / genetics
  • Leukemia, T-Cell / microbiology
  • Leukemia, T-Cell / veterinary*
  • Molecular Sequence Data
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-myc
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Tumor Cells, Cultured

Substances

  • DNA, Viral
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myc

Associated data

  • GENBANK/J04330
  • GENBANK/JO4330