Abstract
In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Neoplasm / metabolism
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Cell Adhesion Molecules / metabolism
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Cell Separation / instrumentation
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Cell Separation / methods*
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Centrifugation
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Epithelial Cell Adhesion Molecule
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Equipment Design
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Fluorescein-5-isothiocyanate / chemistry
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HL-60 Cells
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HeLa Cells
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Humans
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MCF-7 Cells
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Microfluidic Analytical Techniques / instrumentation*
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Microscopy, Fluorescence
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Microspheres
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Optical Tweezers*
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Polystyrenes / chemistry
Substances
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Antigens, Neoplasm
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Cell Adhesion Molecules
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EPCAM protein, human
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Epithelial Cell Adhesion Molecule
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Polystyrenes
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Fluorescein-5-isothiocyanate