Abstract
Enterovirus 71 (EV-A71) is a neurotropic virus that can cause severe complications involving the central nervous system. No effective antiviral therapeutics are available for treating EV-A71 infection and drug discovery efforts are rarely focused to target this disease. Thus, the main goal of this study was to discover existing drugs with novel indications that may effectively inhibit EV-A71 replication and the inflammatory cytokines elevation. In this study, we showed that LiCl, a GSK3β inhibitor, effectively suppressed EV-A71 replication, apoptosis and inflammatory cytokines production (Interleukin 6, Interleukin-1β) in infected cells. Furthermore, LiCl and an immunomodular agent were shown to strongly synergize with each other in suppressing EV-A71 replication. The results highlighted potential new treatment regimens in suppressing sequelae caused by EV-A71 replication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aniline Compounds / administration & dosage*
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Aniline Compounds / pharmacology
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Animals
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Antiviral Agents / administration & dosage*
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Antiviral Agents / pharmacology
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Apoptosis / drug effects
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Cell Line
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Chlorocebus aethiops
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Crotonates
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Cytokines / genetics
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Cytokines / metabolism
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Drug Repositioning
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Drug Synergism
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Drug Therapy, Combination
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Enterovirus A, Human / drug effects*
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Enterovirus A, Human / physiology
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Enterovirus Infections / drug therapy*
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Enterovirus Infections / immunology
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Humans
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Hydroxybutyrates / administration & dosage*
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Hydroxybutyrates / pharmacology
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Lithium Chloride / administration & dosage*
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Lithium Chloride / pharmacology
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Nitriles
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Toluidines
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Vero Cells
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Virus Replication / drug effects
Substances
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Aniline Compounds
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Antiviral Agents
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Crotonates
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Cytokines
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Hydroxybutyrates
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Nitriles
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Toluidines
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teriflunomide
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Lithium Chloride
Grants and funding
This work was supported by the Ministry of Science and Technology (MOST) Grants MOST 101-2811-B-400-023 and BP-101-PP-01 to J.T.-A.H. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.