Vanadate counteracts glucagon effects in isolated rat hepatocytes

Life Sci. 1989;44(20):1491-7. doi: 10.1016/0024-3205(89)90328-7.

Abstract

The incubation of isolated rat hepatocytes with vanadate increased the concentration of fructose 2,6-bisphosphate without modifying 6-phosphofructo-2-kinase activity. Vanadate also reverted and prevented the decrease of fructose 2,6-bisphosphate levels, of the "active" form of the 6-phosphofructo 2-kinase and of the pyruvate kinase activity ratio produced by glucagon, by probably counteracting the increase in cyclic AMP concentration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cyclic AMP / metabolism
  • Fasting
  • Food
  • Fructosediphosphates / metabolism
  • Glucagon / pharmacology*
  • Kinetics
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • Phosphofructokinase-1 / metabolism
  • Phosphorylation
  • Pyruvate Kinase / metabolism
  • Rats
  • Rats, Inbred Strains
  • Vanadates / pharmacology*

Substances

  • Fructosediphosphates
  • Vanadates
  • fructose 2,6-diphosphate
  • Glucagon
  • Cyclic AMP
  • Phosphofructokinase-1
  • Pyruvate Kinase