TPGS-stabilized NaYbF4:Er upconversion nanoparticles for dual-modal fluorescent/CT imaging and anticancer drug delivery to overcome multi-drug resistance

Biomaterials. 2015 Feb:40:107-16. doi: 10.1016/j.biomaterials.2014.11.022. Epub 2014 Nov 26.

Abstract

Multi-drug resistance (MDR) is a major cause of failure in cancer chemotherapy. Tocopheryl polyethylene glycol 1000 succinate (TPGS) has been extensively investigated for overcoming MDR in cancer therapy because of its ability to inhibit P-glycoprotein (P-gp). In this work, TPGS was for the first time used as a new surface modifier to functionalize NaYbF4:Er upconversion nanoparticles (UNCPs) and endowed the as-prepared products (TPGS-UCNPs) with excellent water-solubility, P-gp inhibition capability and imaging-guided drug delivery property. After the chemotherapeutic drug (doxorubicin, DOX) loading, the as-formed composites (TPGS-UCNPs-DOX) exhibited potent killing ability for DOX-resistant MCF-7 cells. Flow-cytometric assessment and Western blot assay showed that the TPGS-UCNPs could potently decrease the P-gp expression and facilitate the intracellular drug accumulation, thus achieving MDR reversal. Moreover, considering that UCNPs process efficient upconversion emission and Yb element contained in UCNPs has strong X-ray attenuation ability, the as-obtained composite could also serve as a dual-modal probe for upconversion luminescence (UCL) imaging and X-ray computed tomography (CT) imaging, making them promising for imaging-guided cancer therapy.

Keywords: Multi-drug resistance; P-gp inhibition; TPGS; Theranostics; Upconversion nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B / metabolism
  • Animals
  • Antineoplastic Agents / pharmacology*
  • Cell Death / drug effects
  • Cell Survival / drug effects
  • Drug Delivery Systems*
  • Drug Resistance, Multiple / drug effects*
  • Fluorescence
  • Humans
  • Luminescence
  • MCF-7 Cells
  • Mice, Nude
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Oleic Acid / chemistry
  • Polyethylene Glycols / pharmacology
  • Spectroscopy, Fourier Transform Infrared
  • Thermogravimetry
  • Tomography, X-Ray Computed*
  • Vitamin E / analogs & derivatives*
  • Vitamin E / pharmacology
  • Ytterbium / chemistry*

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • Antineoplastic Agents
  • Vitamin E
  • Oleic Acid
  • Polyethylene Glycols
  • Ytterbium
  • tocophersolan