Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells

Tiantian Chen; Liyan Yin; Chusen Huang; Yiqiao Qin; Weiping Zhu; Yufang Xu; Xuhong Qian

doi:10.1016/j.bios.2014.11.005

Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells

Biosens Bioelectron. 2015 Apr 15:66:259-65. doi: 10.1016/j.bios.2014.11.005. Epub 2014 Nov 7.

Authors

Tiantian Chen¹, Liyan Yin¹, Chusen Huang², Yiqiao Qin¹, Weiping Zhu³, Yufang Xu¹, Xuhong Qian¹

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
² State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China. Electronic address: [email protected].
³ State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. Electronic address: [email protected].

PMID: 25437361
DOI: 10.1016/j.bios.2014.11.005

Abstract

A naphthalimide-based fluorescent probe CP has been synthesized with simple steps. It can selectively and sensitively recognize copper ions (Cu(2+)) in HEPES buffer (50mM, pH 7.2). The fluorescence intensity of CP is linearly proportional to the concentration of Cu(2+) ranging from 0-8.3μM (correlation coefficient R(2)=0.9808). The resulted complex CP@Cu can serve as a turn-on fluorescent probe for the detection of histidine and histidine rich proteins in broad pH application range. Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200μM (correlation coefficient R(2)=0.9912). Moreover, CP@Cu has potential for imaging histidine in vitro experiments and has promise in real sample applications with great validity.

Keywords: Copper ion; Fluorescent probe; Histidine; Live cell imaging; Naphthalimide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Cell Tracking
Copper / chemistry
Fluorescent Dyes
Histidine / chemistry
Histidine / isolation & purification*
Humans
Molecular Imaging / methods*
Proteins / isolation & purification*

Substances

Fluorescent Dyes
Proteins
Histidine
Copper