Fibroblast Growth Factor-23, Sclerostin, and Bone Microarchitecture in Patients With Osteoporotic Fractures of the Proximal Femur: A Cross-sectional Study

J Clin Densitom. 2016 Apr-Jun;19(2):192-201. doi: 10.1016/j.jocd.2014.09.003. Epub 2014 Nov 8.

Abstract

This cross-sectional observational cohort study was designed to simultaneously investigate bone microarchitecture and serum markers of bone metabolism in elderly osteoporotic patients experiencing a trochanteric or femoral neck fracture. Special emphasis was put on renal function, sclerostin and fibroblast growth factor-23 (FGF-23). Eighty-two patients (median age: 84 years; 49 trochanteric fractures) scheduled for emergency surgery due to an osteoporotic fracture participated. Bone specimens for ex vivo microcomputed X-ray tomography were sampled during surgery. Blood samples for laboratory workup were collected before surgery (t0) and 1 day afterward (t1). Fifty-eight patients consented to dual-energy X-ray absorptiometry scanning of the lumbar spine and/or contralateral femoral neck after recovery during the in-patient stay. Samples were grouped according to the site of fracture. Regression coefficients were controlled for age and/or estimated glomerular filtration rate (eGFR), if appropriate. Patients experiencing a femoral neck fracture presented with better preserved renal function (eGFR) and lower C-terminal fragment of fibroblast growth factor-23 (cFGF-23) concentrations compared to those with trochanteric fractures. By contrast, serum sclerostin was similar at both time points and did not differ between groups. Age-adjusted correlation analysis revealed negative associations between eGFR and cFGF-23 determined at t1 (R=-0.34; p<0.05) as well as between eGFR and sclerostin levels at t0 (R=-0.45; p<0.05) in patients with trochanteric and femoral neck fractures, respectively. Our study provides evidence that not only an age-related decline of renal function but also the type of skeletal injury may contribute to the circulating concentrations of cFGF-23.

Keywords: DXA; osteoporosis; proximal femur fracture; μCT.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorptiometry, Photon / methods
  • Adaptor Proteins, Signal Transducing
  • Aged
  • Aged, 80 and over
  • Bone Density*
  • Bone Morphogenetic Proteins / blood
  • Cohort Studies
  • Cross-Sectional Studies
  • Female
  • Femoral Neck Fractures* / metabolism
  • Femoral Neck Fractures* / pathology
  • Femur Neck / diagnostic imaging
  • Fibroblast Growth Factor-23
  • Fibroblast Growth Factors / blood*
  • Genetic Markers
  • Germany
  • Glomerular Filtration Rate
  • Humans
  • Lumbar Vertebrae / diagnostic imaging
  • Male
  • Osteoporotic Fractures* / metabolism
  • Osteoporotic Fractures* / pathology
  • Risk Factors
  • Statistics as Topic

Substances

  • Adaptor Proteins, Signal Transducing
  • Bone Morphogenetic Proteins
  • FGF23 protein, human
  • Genetic Markers
  • SOST protein, human
  • Fibroblast Growth Factors
  • Fibroblast Growth Factor-23