[Sensitivity of the COBAS AmpliScreen™ HIV-1 test v1.5 for HIV-1 detection]

Rev Argent Microbiol. 2014 Jul-Sep;46(3):196-200. doi: 10.1016/S0325-7541(14)70072-0. Epub 2014 Oct 15.
[Article in Spanish]

Abstract

The introduction of nucleic acid amplification techniques (NAT) in blood banks was intended to reduce the residual risk of transfusion-transmitted infections. Co-circulation of a great diversity of HIV-1 variants in Argentina portrays the need to assess the sensitivity of serological and molecular assays available for their detection. In this study, we evaluated the sensitivity of the COBAS AmpliScreen™ HIV-1 Test, version 1.5 (Roche) for the detection of HIV-1 RNA in plasma samples of infected individuals from Argentina. The results of this study reveal that this technique has high sensitivity for the detection of HIV-1 RNA under assay conditions: using mini-pool testing, pools ≥ 50 RNA copies per ml achieved ≥ 92 % sensitivity, whereas in the standard procedure, samples ≥ 207 RNA copies/ml achieved 100 % sensitivity. Moreover, the COBAS AmpliScreen™ HIV-1 Test, version 1.5 (Roche) is suitable for detecting prevailing HIV-1 variants.

Keywords: COBAS AmpliScreen; HIV-1; NAT.

Publication types

  • Validation Study

MeSH terms

  • Argentina / epidemiology
  • Blood Safety
  • Colorimetry / methods*
  • Genotyping Techniques
  • HIV Infections / blood
  • HIV Infections / epidemiology
  • HIV Infections / virology*
  • HIV-1 / classification
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Mass Screening / methods
  • RNA, Viral / blood*
  • Reagent Kits, Diagnostic
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Ultracentrifugation
  • Viral Load
  • Viremia / virology*

Substances

  • RNA, Viral
  • Reagent Kits, Diagnostic