Site selective activation of lateral hypothalamic mGluR1 and R5 receptors elicits feeding in rats

J R Charles; E Hernandez; A Winter; C R Yang; B G Stanley

doi:10.1016/j.physbeh.2014.10.039

Site selective activation of lateral hypothalamic mGluR1 and R5 receptors elicits feeding in rats

Physiol Behav. 2015 Feb:139:261-6. doi: 10.1016/j.physbeh.2014.10.039. Epub 2014 Nov 18.

Authors

J R Charles¹, E Hernandez², A Winter², C R Yang³, B G Stanley⁴

Affiliations

¹ Department of Cell Biology and Neuroscience, University of California, Riverside, 900 University Ave., Riverside, CA 92521, USA. Electronic address: [email protected].
² Department of Cell Biology and Neuroscience, University of California, Riverside, 900 University Ave., Riverside, CA 92521, USA.
³ Eli Lilly & Co., Lilly Corporate Center, Indianapolis, IN 46285, USA.
⁴ Department of Cell Biology and Neuroscience, University of California, Riverside, 900 University Ave., Riverside, CA 92521, USA; Department of Psychology, University of California, Riverside, 900 University Ave., Riverside, CA 92521, USA.

PMID: 25449406
DOI: 10.1016/j.physbeh.2014.10.039

Abstract

Recent findings from our lab indicate that metabotropic glutamate receptor (mGluR) activation elicits eating, and the goal of the current study was to specify whether the lateral hypothalamus (LH) is the actual brain site mediating this effect. To examine this issue we injected the selective mGluR group I agonist (S)-3,5-dihydroxyphenylglycine (DHPG) unilaterally into the LH and surrounding regions (n=5-8 subjects/brain site) of satiated adult male Sprague-Dawley rats and measured elicited feeding. We determined that 1.0 nmol elicited food intake only within the LH. Increasing the dose to 10 or 25 nmol produced a more sustained effect in the LH, and also elicited eating in several other brain sites. These results, demonstrating that the LH mediates the eating elicited by low doses of DHPG, suggest that the LH may contain mGluR whose activation can produce eating behavior.

Keywords: Feeding; Glutamate; Glutamate receptor; Lateral hypothalamus; Metabotropic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amygdala / drug effects
Amygdala / physiology
Animals
Dose-Response Relationship, Drug
Eating / drug effects
Eating / physiology*
Excitatory Amino Acid Agonists / pharmacology
Glycine / analogs & derivatives
Glycine / pharmacology
Hypothalamic Area, Lateral / drug effects
Hypothalamic Area, Lateral / physiology*
Male
Mediodorsal Thalamic Nucleus / drug effects
Mediodorsal Thalamic Nucleus / physiology
Rats, Sprague-Dawley
Receptor, Metabotropic Glutamate 5 / agonists
Receptor, Metabotropic Glutamate 5 / metabolism*
Receptors, Metabotropic Glutamate / agonists
Receptors, Metabotropic Glutamate / metabolism*
Resorcinols / pharmacology
Satiation / drug effects
Satiation / physiology
Substantia Nigra / drug effects
Substantia Nigra / physiology
Thalamus / drug effects
Thalamus / physiology

Substances

Excitatory Amino Acid Agonists
Grm5 protein, rat
Receptor, Metabotropic Glutamate 5
Receptors, Metabotropic Glutamate
Resorcinols
metabotropic glutamate receptor type 1
3,5-dihydroxyphenylglycine
Glycine