Viral gastroenteritis in rotavirus negative hospitalized children <5 years of age from the independent states of the former Soviet Union

Preeti Chhabra; Elena Samoilovich; Marina Yermalovich; Liudmyla Chernyshova; Stela Gheorghita; Radu Cojocaru; Nazim Shugayev; Gayane Sahakyan; Marina Lashkarashvili; Marina Chubinidze; Khatuna Zakhashvili; Dovile Videbaek; Annemarie Wasley; Jan Vinjé

doi:10.1016/j.meegid.2014.10.013

Viral gastroenteritis in rotavirus negative hospitalized children <5 years of age from the independent states of the former Soviet Union

Infect Genet Evol. 2014 Dec:28:283-8. doi: 10.1016/j.meegid.2014.10.013. Epub 2014 Oct 19.

Affiliations

¹ Division of Viral Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States. Electronic address: [email protected].
² Republican Research and Practical Center for Epidemiology and Microbiology, Ministry of Health, Minsk, Belarus.
³ Department of Pediatric Infectious Diseases and Clinical Immunology of National Academy for Post-graduate Education, Kiev, Ukraine.
⁴ National Center for Public Health, Chişinău, Republic of Moldova.
⁵ Azerbaijan Republican Anti-plague Station, Baku, Azerbaijan.
⁶ National Center of Disease Control and Prevention, Ministry of Health, Yerevan, Armenia.
⁷ National Center for Disease Control and Public Health, Tbilisi, Georgia.
⁸ WHO Regional Office for Europe, Vaccine-preventable Diseases & Immunization, Copenhagen, Denmark.
⁹ Division of Viral Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States.

PMID: 25460823
DOI: 10.1016/j.meegid.2014.10.013

Abstract

Purpose: Rotavirus causes nearly 40% of all hospitalizations for AGE among children <5 years of age in the NIS of the former Soviet Union. The etiologic role of other established gastroenteritis viruses in this age group is unknown.

Methods: Laboratory-confirmed rotavirus negative fecal specimens (N=495) collected between January and December 2009 from children in 6 NIS (Armenia, Azerbaijan, Belarus, Georgia, Republic of Moldova and Ukraine) were tested for norovirus, sapovirus, enteric adenovirus and astrovirus by real-time RT-PCR. Genotyping was carried out by sequencing and phylogenetic analysis.

Results: Norovirus, enteric adenovirus, sapovirus and astrovirus were detected in 21.8%, 4.0%, 3.2%, and 1.4% of the rotavirus negative specimens, respectively. Mixed infections were identified in 4.1% of the specimens. Phylogenetic analysis showed co-circulation of several different genotypes with GII.4 Den Haag (2006b) norovirus, GI.2 sapovirus, adenovirus type 41, and astrovirus type 1 causing majority of the infections.

Conclusion: Norovirus, enteric adenovirus, sapovirus and astrovirus account for a significant proportion (30.5%) of AGE in hospitalized children <5 years of age in 6 NIS.

Keywords: Adenovirus; Astrovirus; Gastroenteritis; Norovirus; Rotavirus; Sapovirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviridae Infections* / epidemiology
Adenoviridae Infections* / virology
Adenoviridae* / classification
Adenoviridae* / genetics
Child, Preschool
Cohort Studies
Feces / virology
Gastroenteritis* / epidemiology
Gastroenteritis* / virology
Humans
Infant
Infant, Newborn
Phylogeny
RNA Virus Infections* / epidemiology
RNA Virus Infections* / virology
RNA Viruses* / classification
RNA Viruses* / genetics
USSR / epidemiology

Grants and funding

001/WHO_/World Health Organization/International