Effects of ubiquinone derivatives on the mitochondrial unselective channel of Saccharomyces cerevisiae

J Bioenerg Biomembr. 2014 Dec;46(6):519-27. doi: 10.1007/s10863-014-9595-3. Epub 2014 Dec 3.

Abstract

Ubiquinone derivatives modulate the mammalian mitochondrial Permeability Transition Pore (PTP). Yeast mitochondria harbor a similar structure: the respiration- and ATP-induced Saccharomyces cerevisiae Mitochondrial Unselective Channel ( Sc MUC). Here we show that decylubiquinone, a well-characterized inhibitor of the PTP, suppresses Sc MUC opening in diverse strains and independently of respiratory chain modulation or redox-state. We also found that naturally occurring derivatives such as hexaprenyl and decaprenyl ubiquinones lacked effects on the Sc MUC. The PTP-inactive ubiquinone 5 (Ub5) promoted the Sc MUC-independent activation of the respiratory chain in most strains tested. In an industrial strain however, Ub5 blocked the protection elicited by dUb. The results indicate the presence of a ubiquinone-binding site in the Sc MUC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Mitochondria / metabolism*
  • Mitochondrial Membrane Transport Proteins / metabolism*
  • Mitochondrial Permeability Transition Pore
  • Reactive Oxygen Species
  • Saccharomyces cerevisiae / metabolism*
  • Ubiquinone / genetics*
  • Ubiquinone / metabolism*
  • Yeasts

Substances

  • Mitochondrial Membrane Transport Proteins
  • Mitochondrial Permeability Transition Pore
  • Reactive Oxygen Species
  • Ubiquinone
  • Ubiquinone Q1