Improving the detection of IgM antibodies against glycolipids complexes of GM1 and Galactocerebroside in Multifocal Motor Neuropathy using glycoarray and ELISA assays

J Neuroimmunol. 2015 Jan 15:278:159-61. doi: 10.1016/j.jneuroim.2014.11.001. Epub 2014 Nov 8.

Abstract

Antibodies against complexes of GM1:GalC are detected in multifocal motor neuropathy. Previous studies used different techniques, explaining disparities in the results. Antibodies against GM1 and GM1:GalC with different proportions of GalC were measured with both glycoarray and ELISA in 20 multifocal motor neuropathies, and 45 controls. The 1:5 ratio and the 1:1 ratio of GM1:GalC (weight ratio) were respectively the most effective for glycoarray and for ELISA. Testing for anti-GM1:GalC antibodies increased the sensitivity from 40% with anti-GM1 antibodies to 65% with array and 60% with ELISA without loss in specificity (above 91%). Anti-GM1:GalC antibodies are effective biological tools to diagnose multifocal motor neuropathy.

Keywords: Anti-ganglioside antibodies; ELISA; Gangliosides complexes; Multifocal motor neuropathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Amyotrophic Lateral Sclerosis / blood*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Galactosylceramides / immunology*
  • Gangliosidosis, GM1 / immunology*
  • Humans
  • Immunoglobulin M / blood*
  • Male
  • Microarray Analysis
  • Middle Aged
  • Polyneuropathies / blood*
  • ROC Curve
  • Statistics, Nonparametric

Substances

  • Galactosylceramides
  • Immunoglobulin M
  • galactocerebroside