Zebrafish embryo intersegmental vessels: a tool for investigating sprouting angiogenesis

Chiara Tobia; Giuseppina Gariano; Jessica Guerra; Marco Presta

doi:10.1007/978-1-4939-1462-3_10

Zebrafish embryo intersegmental vessels: a tool for investigating sprouting angiogenesis

Methods Mol Biol. 2015:1214:173-84. doi: 10.1007/978-1-4939-1462-3_10.

Authors

Chiara Tobia¹, Giuseppina Gariano, Jessica Guerra, Marco Presta

Affiliation

¹ Department of Molecular and Translational Medicine, Experimental Oncology and Immunology, School of Medicine, University of Brescia, Brescia, Italy.

PMID: 25468604
DOI: 10.1007/978-1-4939-1462-3_10

Abstract

Angiogenesis leads to the formation of the intersegmental vessels (ISVs) of the trunk in teleost zebrafish (Danio rerio) embryo. Here we describe experimental procedures, including in vivo observation of transgenic zebrafish embryo and whole mount in situ hybridization, to investigate ISV development in zebrafish embryos and assess the effect of antiangiogenic compounds on these vessels.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified
Blood Vessels / embryology*
Blood Vessels / physiology*
Embryo, Nonmammalian / blood supply*
In Situ Hybridization / methods*
Neovascularization, Physiologic*
Zebrafish / embryology*
Zebrafish / genetics