An adaptive design to investigate the effect of ketoconazole on pharmacokinetics of GSK239512 in healthy male volunteers

J Clin Pharmacol. 2015 May;55(5):505-11. doi: 10.1002/jcph.441. Epub 2015 Feb 4.

Abstract

This open label drug-drug interaction (DDI) study investigated the effect of a strong CYP3A inhibitor ketoconazole on the PK and safety profile of GSK239512. To mitigate the tolerability concerns of high GSK239512 exposures resulting from CYP3A inhibition, a 2-cohort adaptive design was used to facilitate a stepwise selection of dose levels and subject numbers. In Cohort 1, 6 subjects received a single dose of 20 μg GSK239512 alone and then 10 μg GSK239512 in combination with repeated once daily doses of 400 mg ketoconazole. The results from Cohort 1 demonstrated an approximately 1.5-fold increase in GSK239512 exposure with a good tolerability profile. This led to the adoption of a 3-session option in Cohort 2, in which 16 subjects received sequential single doses of 20 μg GSK239512 alone, 40 μg GSK239512 alone, and a single dose of 40 μg GSK239512 in combination with repeated once daily doses of 400 mg ketoconazole. The 2-cohort adaptive design proved effective in mitigating any potentially significant DDI risk to healthy subjects. Final results showed a 1.3-fold increase in GSK239512 exposure with ketoconazole, suggesting that in vivo metabolism of GSK239512 by CYP3A is unlikely to be the primary route of GSK239512 elimination.

Keywords: GSK239512; H3 receptor antagonist; drug-drug interaction; ketoconazole; pharmacokinetics.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Area Under Curve
  • Benzazepines / administration & dosage
  • Benzazepines / pharmacokinetics*
  • Cytochrome P-450 CYP3A Inhibitors / pharmacology*
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Half-Life
  • Healthy Volunteers
  • Histamine Antagonists / administration & dosage
  • Histamine Antagonists / pharmacokinetics*
  • Humans
  • Ketoconazole / pharmacology*
  • Male
  • Metabolic Clearance Rate
  • Middle Aged
  • Research Design*
  • Young Adult

Substances

  • Benzazepines
  • Cytochrome P-450 CYP3A Inhibitors
  • GSK239512
  • Histamine Antagonists
  • Ketoconazole