Next-generation sequencing of the mitochondrial genome and association with IgA nephropathy in a renal transplant population

Adam P Douglas; Dwaine R Vance; Elaine M Kenny; Derek W Morris; Alexander P Maxwell; Amy Jayne McKnight

doi:10.1038/srep07379

Next-generation sequencing of the mitochondrial genome and association with IgA nephropathy in a renal transplant population

Sci Rep. 2014 Dec 9:4:7379. doi: 10.1038/srep07379.

Authors

Adam P Douglas¹, Dwaine R Vance¹, Elaine M Kenny², Derek W Morris², Alexander P Maxwell³, Amy Jayne McKnight¹

Affiliations

¹ Centre for Public Health, Queen's University Belfast, United Kingdom.
² TrinSeq, Trinity Genome Sequencing Lab, Department of Psychiatry, Institute of Molecular Medicine, Trinity College, Dublin, Ireland.
³ 1] Centre for Public Health, Queen's University Belfast, United Kingdom [2] Regional Nephrology Unit, Belfast City Hospital, Belfast, United Kingdom.

Abstract

Kidneys are highly aerobic organs that are critically dependent on the normal functioning of mitochondria. Genetic variations disrupting mitochondrial function are associated with multifactorial disorders including kidney disease. This study sequenced the entire mitochondrial genome in a renal transplant cohort of 64 individuals, using next-generation sequencing, to evaluate the association of genetic variants with IgA nephropathy and end-stage renal disease (ESRD, n = 100).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Gene Frequency
Genes, Mitochondrial
Genetic Association Studies*
Genome, Mitochondrial*
Genomics
Genotype
Glomerulonephritis, IGA / epidemiology
Glomerulonephritis, IGA / etiology*
High-Throughput Nucleotide Sequencing
Humans
Kidney Failure, Chronic / epidemiology
Kidney Failure, Chronic / genetics
Kidney Failure, Chronic / therapy
Kidney Transplantation*
Linkage Disequilibrium
Polymorphism, Single Nucleotide
Reproducibility of Results