DNA-templated silver nanoclusters for multiplexed fluorescent DNA detection

Ying Zhang; Changfeng Zhu; Lei Zhang; Chaoliang Tan; Jian Yang; Bo Chen; Lianhui Wang; Hua Zhang

doi:10.1002/smll.201402044

DNA-templated silver nanoclusters for multiplexed fluorescent DNA detection

Small. 2015 Mar 25;11(12):1385-9. doi: 10.1002/smll.201402044. Epub 2014 Dec 10.

Authors

Ying Zhang¹, Changfeng Zhu, Lei Zhang, Chaoliang Tan, Jian Yang, Bo Chen, Lianhui Wang, Hua Zhang

Affiliation

¹ Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials, National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing, 210023, P. R. China; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.

PMID: 25491417
DOI: 10.1002/smll.201402044

Abstract

Novel label-free/conjugation-free molecular beacons are designed based on DNA templated-silver nanoclusters for multiplexed DNA detection. The assay is implemented in solution, which makes it easy for the in-situ and real-time analysis. This study demonstrates a new method for multiplexd detection of biological molecules by using fluorescent Ag nanocluster-based molecular beacon probes.

Keywords: DNA; fluorescence; multiplexed detection; sensors; silver nanoclusters.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA / analysis
DNA / chemistry*
DNA / genetics*
In Situ Hybridization, Fluorescence / methods*
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Molecular Imprinting / methods*
Molecular Sequence Data
Nanocomposites / chemistry
Nanocomposites / ultrastructure
Reproducibility of Results
Sensitivity and Specificity
Silver / chemistry*
Surface Properties

Substances

Silver
DNA