Abstract
The adrenergic and T3 modulation of UCP expression in non-proliferative foetal brown adipocyte primary cultures were studied. The UCP in the cultured cells was determined by immunological detection of the protein and by quantification of the mitochondrial GDP-binding. Our results showed a relative increase of 65-75% in UCP levels and 60-80% in the mitochondrial GDP-binding capacity under beta-adrenergic stimulatory conditions, while neither alpha 1-adrenergic agonists nor T3 showed an effect.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue, Brown / embryology*
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Adipose Tissue, Brown / metabolism
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Animals
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Carrier Proteins*
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Cells, Cultured
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Energy Metabolism
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Guanosine Diphosphate / metabolism
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In Vitro Techniques
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Ion Channels
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Isoproterenol / pharmacology
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Membrane Proteins / metabolism*
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Mitochondria / metabolism*
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Mitochondrial Proteins
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Rats
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Receptors, Adrenergic, beta / physiology
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Triiodothyronine / pharmacology
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Uncoupling Protein 1
Substances
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Carrier Proteins
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Ion Channels
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Membrane Proteins
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Mitochondrial Proteins
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Receptors, Adrenergic, beta
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Uncoupling Protein 1
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Triiodothyronine
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Guanosine Diphosphate
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Isoproterenol