Abstract
Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay. The development of an immunoassay for detection of serotype 9 rotaviruses should facilitate epidemiologic studies.
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / immunology*
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Antibodies, Viral / immunology
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Antigens, Viral / immunology
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Capsid / immunology*
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Capsid Proteins*
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Enzyme-Linked Immunosorbent Assay
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Female
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Humans
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Hybridomas
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Mice
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Mice, Inbred BALB C
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Neutralization Tests
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Rotavirus / immunology
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Rotavirus / isolation & purification*
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Antigens, Viral
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Capsid Proteins
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VP7 protein, Rotavirus