Abstract
Highly purified human tonsillar B lymphocytes at different stages of activation were incubated with leukotriene B4 (LTB4). As a key marker for activation, we used the CD23 Ag. LTB4 enhanced the CD23 expression on resting B cells in synergy with B cell-stimulating factors from 4% to 50%. Maximal effect of LTB4 was observed at 10(-10) M to 10(-12) M. LTB4 also augmented the S and M phase entries as well as Ig secretion in synergy with IL-2 and IL-4. In contrast, 5S,12S-dihydroxyeicosatetraenoic acid, an isomer of LTB4, and leukotriene C4 lacked these effects. The results indicate that LTB4 amplifies lymphokine-driven activation, replication, and differentiation of human B lymphocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / pharmacology*
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Antigens, Differentiation, B-Lymphocyte / metabolism
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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B-Lymphocytes / physiology
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Cell Differentiation / drug effects
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Cell Division / drug effects
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Child, Preschool
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DNA / biosynthesis
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Humans
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Immunoglobulin G / biosynthesis
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Immunoglobulin M / biosynthesis
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Interphase / drug effects
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Leukocyte Count / drug effects
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Leukotriene B4 / pharmacology*
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Lymphocyte Activation / drug effects*
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Receptors, Fc / metabolism
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Receptors, IgE
Substances
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Adjuvants, Immunologic
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Antigens, Differentiation, B-Lymphocyte
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Immunoglobulin G
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Immunoglobulin M
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Receptors, Fc
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Receptors, IgE
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Leukotriene B4
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DNA