Abstract
Ricin and modeccin do not affect the total number of ribosomes to which phenylalanyl-tRNA becomes bound in the EF 1-dependent reaction. Previous inconsistencies resulted from the use of the nitrocellulose-filter technique, which overestimates the number of control ribosomes engaged in the binding reaction if trace amounts of EF 2 contaminate the ribosomal preparations.
MeSH terms
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Lectins / pharmacology*
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Peptide Elongation Factors / metabolism*
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Phenylalanine
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RNA, Transfer, Amino Acyl / metabolism*
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Ribosome Inactivating Proteins, Type 2
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Ribosomes / drug effects
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Ribosomes / metabolism*
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Ricin / pharmacology*
Substances
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Lectins
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Peptide Elongation Factors
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RNA, Transfer, Amino Acyl
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Ribosome Inactivating Proteins, Type 2
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Phenylalanine
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modeccin
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Ricin