Anti-cancer, pharmacokinetics and tumor localization studies of pH-, RF- and thermo-responsive nanoparticles

N Sanoj Rejinold; Reju George Thomas; Muthunarayanan Muthiah; K P Chennazhi; K Manzoor; In-Kyu Park; Yong Yeon Jeong; R Jayakumar

doi:10.1016/j.ijbiomac.2014.11.044

Anti-cancer, pharmacokinetics and tumor localization studies of pH-, RF- and thermo-responsive nanoparticles

Int J Biol Macromol. 2015 Mar:74:249-62. doi: 10.1016/j.ijbiomac.2014.11.044. Epub 2014 Dec 17.

Authors

N Sanoj Rejinold¹, Reju George Thomas², Muthunarayanan Muthiah³, K P Chennazhi¹, K Manzoor¹, In-Kyu Park⁴, Yong Yeon Jeong², R Jayakumar⁵

Affiliations

¹ Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
² Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju 501-746, South Korea.
³ Department of Biomedical Science and BK21 PLUS Center for Creative Biomedical Scientists, Chonnam National University Medical School, Gwangju 501-746, South Korea.
⁴ Department of Biomedical Science and BK21 PLUS Center for Creative Biomedical Scientists, Chonnam National University Medical School, Gwangju 501-746, South Korea. Electronic address: [email protected].
⁵ Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India. Electronic address: [email protected].

PMID: 25526695
DOI: 10.1016/j.ijbiomac.2014.11.044

Abstract

The curcumin-encapsulated chitosan-graft-poly(N-vinyl caprolactam) nanoparticles containing gold nanoparticles (Au-CRC-TRC-NPs) were developed by ionic cross-linking method. After "optimum RF exposure" at 40 W for 5 min, Au-CRC-TRC-NPs dissipated heat energy in the range of ∼42°C, the lower critical solution temperature (LCST) of chitosan-graft-poly(N-vinyl caprolactam), causing controlled curcumin release and apoptosis to cancer cells. Further, in vivo PK/PD studies on swiss albino mice revealed that Au-CRC-TRC-NPs could be sustained in circulation for a week with no harm to internal organs. The colon tumor localization studies revealed that Au-CRC-TRC-NPs were retained in tumor for a week. These results throw light on their feasibility as multi-responsive nanomedicine for RF-assisted cancer treatment modalities.

Keywords: Chitosan-graft-PNVCL; Curcumin; Gold; RF therapy; Tumor localization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents, Phytogenic / administration & dosage*
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects
Caprolactam / chemistry*
Cell Line, Tumor
Chitosan / chemistry*
Curcumin / administration & dosage*
Curcumin / pharmacology*
Disease Models, Animal
Drug Delivery Systems
Gold / chemistry
Humans
Hydrogen-Ion Concentration
Mice
Nanoparticles / chemistry*
Neoplasms / pathology
Neoplasms / therapy
Particle Size
Pulsed Radiofrequency Treatment
Thermodynamics
Tissue Distribution
Tumor Burden / drug effects
Tumor Burden / radiation effects

Substances

Antineoplastic Agents, Phytogenic
Caprolactam
Gold
Chitosan
Curcumin