Mitochondrial calcium handling in normotensive and spontaneously hypertensive rats: correlation with systolic blood pressure levels

Alejandro Ciocci Pardo; Gustavo J Rinaldi; Susana M Mosca

doi:10.1016/j.mito.2014.12.003

Mitochondrial calcium handling in normotensive and spontaneously hypertensive rats: correlation with systolic blood pressure levels

Mitochondrion. 2015 Jan:20:75-81. doi: 10.1016/j.mito.2014.12.003. Epub 2014 Dec 18.

Authors

Alejandro Ciocci Pardo¹, Gustavo J Rinaldi¹, Susana M Mosca²

Affiliations

¹ Centro de Investigaciones Cardiovasculares, CCT-CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
² Centro de Investigaciones Cardiovasculares, CCT-CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina. Electronic address: [email protected].

PMID: 25530492
DOI: 10.1016/j.mito.2014.12.003

Abstract

The aim was to study the mitochondrial Ca(2+) handling of mitochondria isolated from normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) hearts and to establish a possible correlation with systolic blood pressure (SBP). Mitochondrial swelling after Ca(2+) addition, Ca(2+)-retention capacity (CRC) by calcium green method, and membrane potential (ΔΨm) were assessed. SBP was 124±1 (WKY) and 235±6mmHg (SHR). CRC, Ca(2+) response and ΔΨm were lower in SHR than WKY mitochondria. The conclusion is: the more depolarized state of SHR than WKY mitochondria results in an abnormal Ca(2+) handling and this event is closely associated with the SBP.

Keywords: Ca(2+) response; Ca(2+)-retention capacity; SHR; WKY; mPTP; ΔΨm.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood Pressure*
Calcium / metabolism*
Membrane Potential, Mitochondrial / drug effects
Mitochondria / drug effects
Mitochondria / metabolism*
Rats, Inbred SHR
Rats, Inbred WKY

Substances

Calcium